Reporter

Part:BBa_K3145001:Design

Designed by: Yazmin Camacho   Group: iGEM19_US_AFRL_CarrollHS   (2019-09-09)

sfGFP (Super-Folder Green Fluorescent Protein)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There is C-terminal strep tag

Source

The sfGFP sequence originated from our original pY71-sfGFP construct which was made using the Kwon & Jewett 2015 journal article in our references.

References

  • Kwon, Y.-C. & Jewett, M.C.High-throughput preparation methods of crude extract for robust cell-free protein synthesis. Sci. Rep. 5,8663; DOI:10.1038/srep08663 (2015).